Information on using the quantitect multiplex pcr norox kit with the lightcycler 2. How to use this manual this manual contains all the information you need to operate your minicycler safely and productively. Aug 23, 20 for todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected. Thermal cyclers are used to amplify segments of dna via the polymerase chain reaction. Product specification page 1 96 well lightcycler plate product description order number 72. Realtime pcr was done using 30 ng of human dna in each of the 96 block positions. The lightcycler 96 is a realtime pcr cycler for medium sample throughput. Product specification page 1 96 well lightcycler plate. Roche lightcycler 96 biotecon diagnostics biotecon. Oligo probe 1 is labeled at the 3 end with a donor reporter and oligo probe 2 is labeled at its 5 end with one of a few available acceptor reporters. Flexcycler2 pcr thermal cyclers, analytik jena vwr.
Contact your roche representative for further information. A multitude of different layouts is available for configuration. However, roche diagnostics gmbh reserves the right to make any changes necessary without notice as part of ongoing product development. View and download lg 26lc2r series owners manual online. Press the eject button and wait until the drawer completely opens. Service manual light cycler please note that this is only. The most popular version of this product among our users is 1. The lightcycler instrument of roche molecular biochemicals, mannheim, ge rmany, is a thermocycler for the rapid analysis of pcr applications. For todays academic researcher labs, the new roche lightcycler 96 system is the optimal midsize, midthroughput realtime pcr solution that conveniently delivers the highest standards expected from a lightcycler system. The realtime ready focus panels are designed for expression profiling of genes from a variety of. Chapters 35 describe the basics of installing and operating the minicycler.
System component 2 capillaries, 96 capillaries and capsbox 32 centrifuge adapters with 1 cooling block in an aluminium cooling block lightcycler operator. Biorad mycycler thermal cycler marshall scientific. Direct questions or concerns regarding the contents of this document to. This operators manual contains all the necessary technical information required. Lightcycler 480 instrument operators manual is correct at the time of printing. The cyclers feature a large builtin graphical display for easy selection and monitoring of functions. This manual contains programming information for the agilent technologies n3301a, n3302a, n3303a, n3304a, n3305a, n3306a, and n3307a electronic load modules when installed in an agilent technologies n3300a and n3301a electronic load mainframes. Small footprint quiet enough to be positioned in the bar contemporary stainless steel finish blends right in. Make sure notch on plate is in the bottom right corner. A lightcycler probe system consists of a pair of singlestranded fluorescentlabeled oligonucleotides. Ii contact addresses manufacturer roche diagnostics ltd.
The core algorithm was developed by the computer film company now framestore and has been further developed. Service manual light cycler please note that this is only a. In the context of the seahorse xfp glycolytic rate assay, the process of converting glucose to lactate. Excellent amplification and homogeneity of reaction products. I am using lightcycler 96 for absolute quantification, and want to generate a standard curve, as i am using one reference gene with the 10 different samples with different cdna dilutions such. The lightcycler 96 system embodies roches expertise over the last decade in developing and providing highperformance qpcr systems that enable research breakthroughs. Chapters 6 and 7 describe programming the minicycler. Biorad icycler thermal cycler manual attachments biorad icycler thermal cycler manual 598 kb. Any unauthorized modification of the instrument entails the invalidity of the guarantee. Roche applied science the lightcycler system a proven standard for realtime pcr.
An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user. Buffer capacity of the measurement system, comprising the assay medium and xf assay conditions instrument, sensor, labware. With common well matrixes and high or low profile designs that are compatible with thermal cyclers, the pcr plates facilitate rapid segment amplification when manual processing is not wanted. Compatible to run on any roche or nonroche qpcr insrument. Biorad mycycler thermal cycler the biorad mycycler thermal cycler, 115v, is a sleek 96well pcr system with a gradient feature used for gene amplification. Customers using roche in vitro diagnostic lightcycler kits have to use manual a, marked for in vitro diagnostic use not available in all countries. These adjustments control the calibration and accuracy. Warranty information on warranty conditions are specified in the sales contract. System component 2 lightcycler capillaries 96 capillaries and stoppersbox1 32 lightcycler centrifuge adapters in an aluminum cooling block1 1 lightcycler operators manual 1 lightcycler software package1 cable to connect lightcycler instrument to the computer power cord german power cord u. About this manual the foundry tinder box1 introduction 5 the foundry keylight introduction welcome to this user guide for keylight on after effects.
Power lines an outside antenna system should not be located in the vicinity of. Make sure the correct thermal block cycler 96well for f plate or 384well block format for g plate is in place. The lightcycler 480 system is a highperformance, flexible throughput qpcr platform interchangeable 96 or 384well blocks that provides various methods for gene detection, gene expression. Also used to facilitate other temperaturesensitive reactions, the. At present, one of the main medical problems in hospitals is the increasing number. Owners manual in order to take advantage of the many interesting features that will provide years of enjoyable amateur radio operation. Genemate lightcycler semiskirted, lowprofile, 96well pcr. System component 1 lightcycler instrument sample carousel for o 1. Chapter 2 describes the physical characteristics of the minicycler. The labcycler was developed to operate biochemical thermal processes in tubes of the size 0.
Lightcycler 96 is used by 2 users of software informer. Bottlecyclers chute application alternatively, we can integrate the machine into. The lightcycler 480 ii with led is suitable for detection systems with different probe types such as hybridization probes or hydrolysis 5nuclease taqman probes. We do not recommend performing realtime, multiplex pcr using taqman probes or quantitect assays on the lightcycler system due to the limitations of its optical detection system. Turn on the computer linked to the instrument and log on to window xp. This lightcyler 480 instrument quick guide was created by the roche diagnostics corporation commercial education department. Make sure the correct thermal block cycler 96 well for f plate or 384well block format for g plate is in place. The flexcycler2 thermal cyclers offers stateoftheart heating and cooling rates in combination with efficient control and accuracy. Place plate in drawer and push drawer until it completely closes. Service manual 300 watt single input electronic loads agilent technologies model 6060b and 6063b for instruments with serial numbers agilent 6060b us37350101 and up agilent 6063b us37350101 and up for instruments with higher serial numbers, a change page may be included. Lightcycler 96 realtime pcr system roche life science. The versatile, platebased lightcycler 96 system helps you achieve fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and snp analysis compact and easy to use, the lightcycler 96 system primary is the ideal companion for applications including gene expression analysis, gene detection, mutation detection, methylation analysis. In preparation for real time polymerase chain reaction applications, small segments of dna or rna are isolated and placed in pcr plates. The cycler has 4 detection channels, which detect hydrolysis 5nuclease taqman probes either as single color or multiplex samples.
Roche diagnostics corporation commercial education department 9115 hague road p. Taqman fast advanced master mix on the roche lightcycler. This compact thermal cycler features intuitive programming of temperatures, times, and protocols with readymade templates for ease of viewing, editing, and running protocols. Assays are preplated and dried down for a reaction volume of 20 l on 96well plates and 10 l on 384well plates. Mycycler thermal cycler instruction manual catalog number 1709703 for technical service call your local biorad office or in the u. The free 3 hydroxyl group of probe 2 must be blocked. Alphanumeric grid, colored manufacturing, and the labeling surface. These units will be referred to as electronic load throughout this manual. The lightcycler 480 ii is a realtime pcr cycler for mid to high sample throughput. Agilent seahorse xfp glycolytic rate assay kit user guide 9 glossary glycolysis. At present, one of the main medical problems in hospitals is the increasing number of nosocomial hospitalacquired infections. Keylight is an industryproven blue and green screen keyer.
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